Biofilm forms on the teeth immediately after cleaning. First, a salivary pellicle bind to the teeth, which protects the tooth from external factors such as acidic foods or beverages. The salivary pellicle begins to gather bacteria, which form a biofilm/coating of plaque on the teeth. This plaque thickens over time and after 48 hours a mature biofilm is formed. A mature biofilm contains pathogenic bacteria that already cause oral disease, tooth decay, gingivitis or periodontitis/parodontitis. If this biofilm is not cleaned regularly every day by means of personal oral hygiene, a mature biofilm will build up in your mouth with lots of bacteria. This means that, if you want to have healthy beautiful teeth and gums without inflammation, it is important to thoroughly remove the biofilm from the surface of your teeth every day. Knowing the correct technique and using interdental brushes is a guaranteed success for healthy gums and healthy teeth.